A Comparative Analysis of Metabolome Reveals the Regulation of the Anthocyanin Biosynthesis Branch in Potatoes

Abstract A comparative metabolomic analysis was performed using purple potato tubers and derived red mutant to investigate the regulation of anthocyanin biosynthesis. The metabolome revealed 19 key differential metabolites including 10 flavonoids 4 nucleotides. As a metabolite, pelargonidin responsible for mutant, other had correlation with it. To further analyse regulatory mechanism metabolites, analyses transcriptome were done. Among seven F3?5?H in flavonoid metabolic pathway. is biosynthesis branch tubers. , identified 63 differentially expressed genes, which highly correlated F3'5'H . Furthermore, correlations among genes indicated that 11 presented 76 significant correlations. These mainly encoded serine/threonine protein kinase, UMP-CMP MADS-box transcription factor, WD repeat-containing protein. involved pelargonidin, luteolin, kaempferol-3-O-rutinoside, eriodictyol-7-O-glucoside, uridine monophosphate. Through yeast one-hybrid experiments, proteins interacting promoter screened: ethylene-responsive factor ERF011, gibberellin-regulated 9-like, serine-threonine LHW bHLH. results will provide valuable insights into potatoes.